论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
Authors Liang T, Li L, Cheng Y, Ren C, Zhang G
Received 23 June 2015
Accepted for publication 13 January 2016
Published 15 July 2016 Volume 2016:9 Pages 4307—4315
DOI https://dx.doi.org/10.2147/OTT.S90976
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Ram Prasad
Peer reviewer comments 3
Editor who approved publication: Dr Faris Farassati
Abstract: Ovarian carcinoma is the most lethal gynecologic malignancy among women.
Ovarian cancer metastasis is the main reason for poor prognosis. MicroRNAs
(miRNAs) have been shown to play an important role in tumorigenesis and
metastasis in various cancers by affecting the expression of their targets. In
this study, we explored the role of miR-194 in ovarian cancer. Real-time
polymerase chain reaction assays showed that miR-194 was significantly
upregulated in ovarian cancer tissues. Overexpression of miR-194 in ovarian
cancer cells promotes cell proliferation, migration, and invasion; in contrast,
inhibition of the expression of miR-194 has the opposite effects. Meanwhile,
bioinformatics tools were used to identify protein tyrosine phosphatase
nonreceptor type 12 (PTPN12) as a potential target of miR-194. The luciferase assay
showed that miR-194 directly binds to the 3'-untranslated region of PTPN12.
Western blot analysis and quantitative real-time polymerase chain reaction
assay revealed that PTPN12 expression was negatively associated with miR-194
expression in both ovarian cancer tissues and cells. Thus, we conclude that
miR-194 targets PTPN12 and functions as an oncogene in ovarian cancer cells.
This novel pathway may provide a new insight to explain ovarian cancer
development and metastasis.
Keywords: miR-194, ovarian cancer, PTPN12,
metastasis