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Authors Liang G, Li Y, Feng W, Wang X, Jing A, Li J, Ma K
Received 27 August 2016
Accepted for publication 13 October 2016
Published 15 November 2016 Volume 2016:11 Pages 6079—6088
DOI https://doi.org/10.2147/IJN.S120828
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Akshita Wason
Peer reviewer comments 2
Editor who approved publication: Dr Linlin Sun
Abstract: Quantum dots (QDs) have been intensively investigated for bioimaging,
drug delivery, and labeling probes because of their unique optical properties.
In this study, CdSe/ZnS QDs-based nonviral vectors with the dual functions of
delivering miR-26a plasmid and bioimaging were formulated by capping the
surface of CdSe/ZnS QDs with polyethyleneimine (PEI). The PEI-coated QDs were
capable of condensing miR-26a expression vector into nanocomplexes that can
emit strong red luminescence when loaded with CdSe/ZnS QDs. Further results
showed that PEI-modified nanoparticles (NPs) could transfect miR-26a plasmid
into HepG2 cells in vitro. Meanwhile, imaging of living cells could be achieved
based on the CdSe/ZnS QDs. Further study suggested that miR-26a transfection
up-regulated miR-26a expression, induced cycle arrest, and triggered
proliferation inhibition in HepG2 cells. The results indicated that PEI-coated
QD NPs possess the capability of bioimaging and gene delivery and could be a
promising vehicle with the engineering of QD NPs for gene therapy in the
future.
Keywords: miR-26a, PEI/QDs, HepG2, gene
delivery, bioimaging