论文已发表
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Authors Cui Y, Zhang C, Luo R, Liu H, Zhang Z, Xu T, Zhang Y, Wang D
Received 20 June 2016
Accepted for publication 19 September 2016
Published 14 November 2016 Volume 2016:11 Pages 5671—5682
DOI https://doi.org/10.2147/IJN.S115357
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Eytan Klausner
Peer reviewer comments 2
Editor who approved publication: Dr Linlin Sun
Purpose: Arginine-glycine-aspartic acid (RGD)-based nanoprobes allow specific
imaging of integrin αvβ3, a protein overexpressed during angiogenesis.
Therefore, this study applied a novel RGD-coupled, polyacrylic acid
(PAA)-coated ultrasmall superparamagnetic iron oxide (USPIO) (referred to as
RGD-PAA-USPIO) in order to detect tumor angiogenesis and assess the early
response to antiangiogenic treatment in human nasopharyngeal carcinoma (NPC)
xenograft model by magnetic resonance imaging (MRI).
Materials and methods: The binding specificity of RGD-PAA-USPIO with human
umbilical vein endothelial cells (HUVECs) was confirmed by Prussian blue
staining and transmission electron microscopy in vitro. The tumor targeting of
RGD-PAA-USPIO was evaluated in the NPC xenograft model. Later, mice bearing NPC
underwent MRI at baseline and after 4 and 14 days of consecutive treatment with
Endostar or phosphate-buffered saline (n=10 per group).
Results: The specific uptake of the RGD-PAA-USPIO nanoparticles
was mainly dependent on the interaction between RGD and integrin αvβ3 of
HUVECs. The tumor targeting of RGD-PAA-USPIO was observed in the NPC xenograft
model. Moreover, the T2 relaxation time of mice in the Endostar-treated group
decreased significantly compared with those in the control group both on days 4
and 14, consistent with the immunofluorescence results of CD31 and CD61 (P <0.05).
Conclusion: This study demonstrated that the magnetic resonance
molecular nanoprobes, RGD-PAA-USPIOs, allow noninvasive in vivo imaging of
tumor angiogenesis and assessment of the early response to antiangiogenic
treatment in NPC xenograft model, favoring its potential clinical translation.
Keywords: magnetic resonance imaging, ultrasmall
superparamagnetic iron oxide, integrin αvβ3, antiangiogenic therapy, Endostar,
nasopharyngeal carcinoma