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与氟喹诺酮和氨基糖苷类对鸟分枝杆菌分离株耐药性有关的单核苷酸多态性

 

Authors Pang H, Wan K, Wei L

Received 27 December 2017

Accepted for publication 12 February 2018

Published 9 April 2018 Volume 2018:11 Pages 515—521

DOI https://doi.org/10.2147/IDR.S160899

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 3

Editor who approved publication: Dr Sahil Khanna

Objective: The relationships between fluoroquinolone and aminoglycoside resistance and single-nucleotide polymorphisms (SNPs) in gyrA gyrB , and rpsL  genes were investigated in 95 clinical isolates of Mycobacterium avium  from China. 
Methods: Fluoroquinolone and aminoglycoside resistance were determined by the broth microdilution method. GyrA gyrB , and rpsL  were sequenced, SNPs were identified, and the corresponding amino acid mutations were recorded.
Results: The M. avium  isolates displayed high levels of ofloxacin (93.68%), ciprofloxacin (92.63%), and streptomycin (65.26%) resistance. Moxifloxacin (18.95%) and amikacin (2.11%) were highly active against the strains. Fluoroquinolone resistance involving gyrA  and gyrB  gene mutations was identified. For gyrA , the most frequent SNPs were T
C (71/95, 74.74%), followed by AG (64/95, 67.37%) and TC (62/95, 65.26%). The amino acid mutations occurred mainly at Gly2444Asp (GGTGAT) (20/95, 21.05%), Ala2445Ser (GCCTCC) (20/95, 21.05%), Ala2447Val (GCCGTC) (20/95, 21.05%), Val2449Ile (GTCATC) (20/95, 21.05%), and Glu2450Gln (GAACAA) (20/95, 21.05%). Prominent SNPs in gyrB  included AC (69/95, 72.63%), CT (51/95, 53.68%), and TG (29/95, 30.53%), and their amino acid substitutions were Ile2160Val (ATTGTT) (21/95, 22.11%), Ile2160Met (ATTATG) (20/95, 21.05%), and Ile2273Leu (ATCCTC) (11/95, 11.58%). Among the strains with aminoglycoside resistance, SNPs in rpsL  were identified mostly at position GA (73/95, 76.84%). GC (21/95, 22.11%) was commonly seen. The amino acid mutations primarily involved Ala1539985Thr (GCCACC) (19/95, 20.00%), His1539992Asp (CACGAC) (19/95, 20.00%), and Gln1539983Glu (CAGGAG) (18/95, 18.95%).
Conclusion: Our study provides valuable information that could be used for the future diagnosis and treatment of M. avium  disease.
Keywords: Mycobacterium avium , drug resistance, single-nucleotide polymorphism, amino acid mutation, minimum inhibitory concentration


 

摘要视频链接SNPs related to fluoroquinolone and aminoglycoside resistance